Molecular detection of two strains of Sugarcane yellow leaf virus in India and their secondary spread in nature through aphids

Singh, Deepti and Rao, G. P. (2011) Molecular detection of two strains of Sugarcane yellow leaf virus in India and their secondary spread in nature through aphids. Acta Phytopathologica et Entomologica Hungarica, 46 (1). pp. 17-26. ISSN 0238-1249

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Abstract

Thirteen Sugarcane yellow leaf virus isolates causing yellow midrib and irregular yellow spot patterns from six states of India were characterized by RT-PCR assays. SCYLV-615F and SCYLV-615R primers were used as forward and reverse primer pairs and the amplified products were cloned and sequenced. Comparative coat protein sequence analysis confirmed that all the SCYLV-Indian isolates were clustered into two major groups confirming the existence of two strains of SCYLV affecting sugarcane crops of India. However, all the 13 isolates under study were found related with Cuban isolate and belong to the CUB genotype. We concluded that YLD in India is caused by two genotypes CUB and IND, rather than by three genotypes as reported earlier. In a separate experiment, the member of both of the phylogenetic groups were found to be transmitted by the sugarcane aphid Melanaphis sacchari from infected to healthy sugarcane suggesting its secondary spread in nature.

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