Morphological and molecular identification of airborne Fusarium propagules trapped in a maize field in Hungary

Szécsi, Á. and Koncz, Zsuzsanna and Magyar, D. (2011) Morphological and molecular identification of airborne Fusarium propagules trapped in a maize field in Hungary. Acta Phytopathologica et Entomologica Hungarica, 46 (2). pp. 175-184. ISSN 0238-1249

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Abstract

Airborne propagules of Fusarium spp. were collected on Fusarium selective medium with Andersen sampler in maize field. Fusarium isolates were identified based on morphological characters and using speciesspecific primersVERT 1/2, PRO 1/2 and SUB 1/2. The PCR analysis confirmed morphological identification of F. verticillioides, F. proliferatum and F. subglutinans. The VERTF 1/2 set of primers were used to discriminate potential fumonisin-producing strains of F. verticillioides, and all F. verticillioides strains scored positive for VERTF 1/2 pair of primers. This is the first study where specific primers were used for the confirmation of morhological determination of the above-mentioned Fusarium species, and selection of fumonisin-producing airborne isolates of F. verticillioides in Hungary.

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