Laczkó-Zöld, Eszter and Forgó, Péter and Zupkó, István and Sigrid, Eşianu and Hohmann, Judit (2017) Isolation and quantitative analysis of physalin D in the fruit and calyx of Physalis alkekengi L. Acta Biologica Hungarica, 68 (3). pp. 300-309. ISSN 0236-5383
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Abstract
Physalin D was isolated from the methanol extract of Physalis alkekengi L. fruits by combination of different chromatographic methods (CPC, TLC, HPLC). The structure was elucidated based on <sup>1</sup>H and <sup>13</sup>C NMR spectral analysis with the aid of 2D-correlation spectroscopy (<sup>1</sup>H, <sup>1</sup>H-COSY, HSQC and HMBC) and comparison with literature data. The quantity of physalin D in mature and immature fruits and calyces was determined by RP-HPLC-UV method. Among the studied samples, immature calyx showed the highest content of physalin D (0.7880 ± 0.0612%), while mature calyx contained 4 times less amount (0.2028 ± 0.016%). The physalin D content of the fruit was much lower; immature fruits contained 0.0992 ± 0.0083% physalin D and mature fruits 0.0259 ± 0.0021%. The antiproliferative activity of the CHCl<sub>3</sub> extract and its fractions was tested on three cancer cell lines (HeLa, MCF-7 and A431). The antiproliferative activity of physalin D is discussed with regard the published data.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > QH Natural history / természetrajz |
Depositing User: | Ágnes Sallai |
Date Deposited: | 08 Nov 2017 10:45 |
Last Modified: | 30 Sep 2018 23:16 |
URI: | http://real.mtak.hu/id/eprint/67210 |
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