Differential regulation of the two metallothionein genes in common carp

Hermesz, Edit and P. Gazdag, Anett and K. Said, Ali and Nemcsók, J. and Ábrahám, Magdolna (2002) Differential regulation of the two metallothionein genes in common carp. Acta Biologica Hungarica, 53 (3). pp. 343-350. ISSN 0236-5383

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Abstract

The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.

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