Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay

Djelić, N. and Spremo-Potparević, Biljana and Djelić, Dijana (2005) Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay. Acta Biologica Hungarica, 56 (3-4). pp. 403-406. ISSN 0236-5383

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Abstract

The objective of the present study was to evaluate possible genetic changes in cultured human lympho- cytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concen- trations of estradiol were used (range from 10 –10 M to 0.7 × 10 –4 M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucle- ated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclu- sion, estradiol treatment might represent a human health risk, especially if overdosed or used for a pro- longed period of time.

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