A novel aspect of NADPH production in ageing Penicilliumchrysogenum

Varecza, Z. and Emri, T. and Pusztahelyi, Tünde and Pócsi, I. (2006) A novel aspect of NADPH production in ageing Penicilliumchrysogenum. Acta Biologica Hungarica, 57 (1). pp. 115-121. ISSN 0236-5383

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Abstract

NADPH is involved in many basically important anabolic processes. For a long time, pentose phosphate pathway (PPS) was regarded as the most important source of NADPH in fungi. Here we present evidence of a metabolic switch to an alternative NADPH-producing pathway in ageing Penicillium chrysogenum cultures, which involves NADP + -specific isocitrate dehydrogenase (NADP + -ID) rather than PPS enzymes. Considering the main biochemical functions of NADPH, we propose that NADP + -ID could have deep impact on many physiological processes switched on glucose deprivation including proteinase production or penicillin biosynthesis. We also demonstrate that although the alternative pathway was inferior to PPS when the fungus was grown on well-utilisable carbon sources yet it could have an impor- tant role in fatty acid biosynthesis as well as in the maintenance of high intracellular NADPH/NADP + ratios.

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