Bokor, Z. and Müller, T. and Bercsényi, M. and Horváth, L. and Urbányi, B. and Horváth, Á. (2007) Cryopreservation of sperm of two European percid species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis). Acta Biologica Hungarica, 58 (2). pp. 199-207. ISSN 0236-5383
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Abstract
Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis) . Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fertilization. In the second, the effects of two dilution ratios (1: 1, 1: 9) and two cryoprotectants (DMSO, MeOH) on hatching were investigated. In the experiment on Volga pikeperch the suitability of using cryopreservation for fertilization was investigated. In the first experiment on pikeperch the highest post-thaw motility (28 ± 21%) and fertilization rate (43 ± 12%) was found with DMSO as cryoprotectant in combination with Glucose extender. In the second, the highest hatch rate (41 ± 22%) was observed with MeOH as cryoprotectant and 1: 1 sperm dilution ratio, however no significant difference was found among the results. In the experiment on Volga pikeperch hatch rates with cryopreserved sperm (60 ± 2%) did not significantly differ from the control (60 ± 6%). Contamination of sperm with urine seems to be a key problem in the success of sperm cryopreservation of these species.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > QH Natural history / természetrajz |
Depositing User: | Endre Sarvay |
Date Deposited: | 20 Nov 2017 15:16 |
Last Modified: | 20 Nov 2017 15:16 |
URI: | http://real.mtak.hu/id/eprint/70122 |
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