High production of (2 S ,3 S )-3-hydroxy-2-methylbutanoate by immobilized plant cells of Marchantia polymorpha

Shimoda, K. and Kubota, N. and Nakajima, N. and Hamada, H. and Hamada, H. (2007) High production of (2 S ,3 S )-3-hydroxy-2-methylbutanoate by immobilized plant cells of Marchantia polymorpha. Acta Biologica Hungarica, 58 (2). pp. 219-223. ISSN 0236-5383

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Abstract

Cultured plant cells of Marchantia polymorpha were examined for their ability to reduce β-keto ester, 2-methyl-3-oxobutanoate. The cells reduced ethyl 2-methyl-3-oxobutanoate to predominantly yield the anti -product, ethyl (2 S ,3 S )-3-hydroxy-2-methylbutanoate, with 92% diastereomeric excess and over 99% enantiomeric excess. The use of immobilized cells of M. polymorpha in calcium alginate gel improved the diastereomeric excess of the product (97% de). In addition, the large-scale reduction of 75 g of ethyl 2-methyl-3-oxobutanoate with immobilized M. polymorpha gave the product with 97% de and >99% ee in 92% yield.

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