EFFECT OF COPPER EXCESS ON H 2 O 2 ACCUMULATION AND PEROXIDASE ACTIVITIES IN BEAN ROOTS

Bouazizi, Houda and Jouili, Hager and Geitmann, Anja and Ferjani, E. (2008) EFFECT OF COPPER EXCESS ON H 2 O 2 ACCUMULATION AND PEROXIDASE ACTIVITIES IN BEAN ROOTS. Acta Biologica Hungarica, 59 (2). pp. 233-245. ISSN 0236-5383

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Abstract

We studied oxidative stress and peroxidase activity resulting from application of excess copper in the nutrient medium on the roots of young bean seedlings. The change in H 2 O 2 content, lipid peroxidation and antioxidant enzymes activities were quantified and located. Excess of copper caused a loss of mem- brane integrity and the formation of hydrogen peroxide (H 2 O 2 ) as visualized in the transmission electron microscopy and measured using spectrophotometry. H 2 O 2 accumulated in the intercellular spaces and in the cell wall. The production of H 2 O 2 was accompanied by an increase in the activity of soluble and ionic GPX (guaiacol peroxidase, EC 1.11.17), CAPX (coniferyl alcohol peroxidase) and NADH oxidase.

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