Preliminary studies on the effect of zinc and selenium on vanadium-induced cytotoxicity in vitro

Zwolak, Iwona and Zaporowska, Halina (2009) Preliminary studies on the effect of zinc and selenium on vanadium-induced cytotoxicity in vitro. Acta Biologica Hungarica, 60 (1). pp. 55-67. ISSN 0236-5383

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Abstract

In the present work, we investigated the cytotoxicity of vanadium and the influence of zinc and seleni- um on vanadium-dependent cell damage in the BALB/c 3T3 cell culture. Treatment of cells for 24 hours with medium containing 50, 100 and 200 μ M NaVO 3 caused a significant decrease in the cell viability as measured by MTT test. Furthermore, the assays for reactive oxygen species (NBT reduction and phe- nol red oxidation) demonstrated the increase in superoxide and hydrogen peroxide production. In the cotreatment studies, the cells were exposed to NaVO 3 (50, 100 and 200 μ M) in the presence of nontox- ic concentrations of ZnCl 2 (5 μ M) or Na 2 SeO 3 (0.5 μ M). Following 24 h incubation, the cell viability (assessed in MTT assay) and reactive oxygen species generation were evaluated. Our data suggest that zinc and selenium, in the concentrations mentioned above, provide no protection against adverse actions induced by sodium metavanadate at concentration levels of 50, 100 and 200 μ M. To our knowledge, this is the first report from in vitro studies on interaction between pentavalent vanadium and trace elements that function as antioxidants: zinc and selenium.

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