Shoot development from hypocotyl protoplasts of sunflower ( Helianthus annuus L.)

Taski-Ajdukovic, Ksenija and Nagl, Nevena and Miladinovic, Dragana and Mikic, A. (2009) Shoot development from hypocotyl protoplasts of sunflower ( Helianthus annuus L.). Acta Biologica Hungarica, 60 (2). pp. 233-239. ISSN 0236-5383

[img] Text
Restricted to Repository staff only until 30 June 2029.

Download (1MB)


The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated microcalluses to solid regeneration media. Shoot regeneration was achieved by culture of calluses on SE regeneration medium after the treatment with 2.2 μM thidiazuron.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 23 Nov 2017 14:34
Last Modified: 23 Nov 2017 14:34

Actions (login required)

Edit Item Edit Item