Said Ali, K. and Ferencz, Ágnes and Nemcsók, J. and Hermesz, Edit (2010) Expressions of heat shock and metallothionein genes in the heart of common carp ( Cyprinus carpio ): Effects of temperature shock and heavy metal exposure. Acta Biologica Hungarica, 61 (1). pp. 10-23. ISSN 0236-5383
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Abstract
Heat shock proteins (HSPs) and metallothioneins (MTs) play important roles in protection against environmental stressors. The present study analyzes and compares the regulation of heat shock (hsp70, hsc70- 1 and hsp90α) and metallothionein (MT-1 and MT-2) genes in the heart of common carp, in response to elevated temperature, cold shock and exposure to several heavy metal ions (As3+, Cd2+ and Cu2+), in whole-animal experiments. Among these metals, arsenate proved to be the most potent inducer of the examined stress genes; the hsp90α and MT-1 mRNA levels were elevated 11- and 10-fold, respectively, after a 24-h exposure. In contrast, Cd2+ at 10 mg/L had no impact on the expression of hsp90α, and the MT genes also proved to be rather insensitive to Cd2+ treatment in the heart: only a 2–2.5-fold induction was observed in response to 10 mg/L Cd2+. Heat shock resulted in a transient induction of hsp70 (19- fold) and hsp90α (15-fold), while elevated temperature had no effect on the expression of the MTs. Direct cold shock induced hsp70 expression (14-fold), while the hsp90α (26-fold) and MT-2 (2-fold) expressions peaked after the recovery period following a direct cold shock. The five stress genes examined in this study exhibited a unique, tissue-specific basal expression pattern and a characteristic sensitivity to metal treatments and temperature shocks.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > QH Natural history / természetrajz |
Depositing User: | Endre Sarvay |
Date Deposited: | 23 Nov 2017 15:05 |
Last Modified: | 23 Nov 2017 15:05 |
URI: | http://real.mtak.hu/id/eprint/70364 |
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