Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra

Chmielarz, P. (2010) Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra. Acta Biologica Hungarica, 61 (2). pp. 224-233. ISSN 0236-5383

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Abstract

In order to evaluate the feasibility of cryopreservation of Wych elm (Ulmus glabra Huds.) seeds, we evaluated the seeds sensitivity to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; –196 °C). We also determined the critical water content (WC) of desiccated seeds and the highmoisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or up to two years in LN. Germination tests revealed no critical WC for seeds to 0.03 g H2O g–1 dry mass, g g–1. Seeds tolerated freezing in LN within safe ranges of WC 0.03–0.21 g g–1 (nuts). Seeds desiccated to the safe WC and stored in LN for two years had similar germination as seeds stored at –3 °C for two years. Therefore, long-term cryopreservation of U. glabra seeds in gene banks is feasible.

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