Influence of genotype and explant source on the in vitro regeneration ability of different melon varieties

Kiss-Bába, Erzsébet and Pánczél, Sarolta and Velich, I. and Bisztray, G. (2010) Influence of genotype and explant source on the in vitro regeneration ability of different melon varieties. Acta Biologica Hungarica, 61 (4). pp. 498-511. ISSN 0236-5383

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Abstract

Nine genotypes of melon (Cucumis melo L.) were selected for the investigation of regeneration. Most of the tested varieties showed regeneration ability on medium containing 0.5 mg l –1 or 1 mg l –1 BA, but following the appearance of shoot buds, only six varieties produced leafy shoots. The effect of combinations of BA with different auxins (IAA, NAA, 2,4-D) and ABA in the culture medium on shoot regeneration was tested on cotyledon explants of ‘Hógolyó’ and ‘Hale’s Best’. To establish optimal conditions for the adventitious shoot induction six types of seedling-derived explants were prepared from seedlings of four different ages. The best results for shoot forming capacity were achieved with cotyledons followed by decapitated seedlings and hypocotyls derived from 4-day-old seedlings. Cotyledon segments of ‘Hógolyó’ and ‘Hale’s Best’ were also cultivated on media with different concentrations of IAA and BA supplemented with 0.26 mg l –1 ABA. The highest number of well-formed plantlets was counted for ‘Hógolyó’ on the medium supplemented with 0.9 mg l –1 BA +0.6 mg l –1 IAA +0.26 mg l –1 ABA. This is the first report on the in vitro regeneration of ‘Hógolyó’ from decapitated seedling and hypocotyl explants and of ‘Javított Zentai’, ‘Muskotály’, ‘Hógolyó’, ‘Tétényi csereshéjú’ and ‘Magyar Kincs’ from cotyledon explants.

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