In vitro shoot proliferation of Telekia speciosa (Schreb.) Baumg. Induced by different cytokinins

Csabai, Judit and Nagy, Z. and Mándy, Aandrea (2011) In vitro shoot proliferation of Telekia speciosa (Schreb.) Baumg. Induced by different cytokinins. Acta Biologica Hungarica, 62 (4). pp. 453-462. ISSN 0236-5383

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Abstract

In vitro shoot multiplication of Telekia speciosa (Schreb.) Baumg. was tested on media containing benzyladenine, benzyladenine riboside, kinetin, zeatin, meta-topolin or 2-isopentenyladenine in different concentrations. We observed the proliferation rate, the length of shoots, rate of callus formation, and the presence of the hyperhydricity. The highest proliferation rate (13.17) was obtained on medium supplemented with 5.0 μM benzyladenine, however, the leaves were hyperhydrated at this concentration of benzyladenine, therefore for shoot multiplication lower (1.0 μM BA) concentration of benzyladenine is suggested. The longest shoots were achieved using 1.0 μM 2-iP. At this treatment 100% rooting was found, therefore the stage of rooting is omissible using 1.0 μM 2-iP during the multiplication. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this plant species.

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