Fábián, Renáta and Kovács, András and Stéger, Viktor and Frank, Krisztián and Egerszegi, István and Oláh, János and Bodó, Szilárd (2017) X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats. Acta Veterinaria Hungarica, 65 (4). pp. 500-504. ISSN 0236-6290
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Abstract
The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
Item Type: | Article |
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Subjects: | S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány |
Depositing User: | Erika Bilicsi |
Date Deposited: | 03 Jan 2018 20:16 |
Last Modified: | 31 Dec 2018 00:18 |
URI: | http://real.mtak.hu/id/eprint/71811 |
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