Ductular reaction at the early terms of common bile duct ligation in the rats

Azmaiparashvili, Elza and Berishvili, Ekaterine and Kakabadze, Z. and Pilishvili, O. and Mikautadze, Ekaterine and Solomonia, R. and Jangavadze, M. and Kordzaia, D. (2012) Ductular reaction at the early terms of common bile duct ligation in the rats. Acta Biologica Hungarica, 63 (3). pp. 321-332. ISSN 0236-5383

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Ductular reaction (DR) in bile duct ligated rats generally appears from 2<sup>nd</sup> day after biliary obstruction (BO). However, we show that increased amount of ductular profiles is evident already in 6 hours after BDL. The study aims to explain the origin of such an early DR in response to BO. Male Lewis rats were subjected to common bile duct ligation (CBDL) for 3, 6, 12 and 24 hours and sham operation. Liver samples were studied histologically, immunohistochemically (Ki67, pan-Cytokeratin /AE1 + AE3/ and OV-6) and by immunoblotting analyses. It appeared that number of ductular profiles increase in timerelated manner after BO. These ductular profiles are formed by biliary epitheliocyte-like cells; No mitotic activity was revealed. Part of hepatocytes reveals pan-Cytokeratin positivity on 12 and 24 hours after BO. Total cytokeratins content at 24 hours after CBDL was 37% higher in comparison with control data. The significant increase was observed for the cytokeratins with molecular weights: 61, 56 and 40 KDa. Thus, early DR after BDL is mediated by widening of the existed finest biliary ramifications and is not associated with proliferation activities. This DR is accompanied by differentiation of hepatocytes toward bile duct-like cells.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Ágnes Sallai
Date Deposited: 12 Jan 2018 07:19
Last Modified: 12 Jan 2018 07:19

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