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Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics

Tóth, Eszter A. and Oszvald, Ádám and Péter, Mária and Balogh, Gábor and Osteikoetxea-Molnár, Anikó and Bozó, Tamás and Szabó-Meleg, Edina and Nyitrai, Miklós and Vigh, László and Matkó, János (2017) Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1862 (9). pp. 991-1000. ISSN 1388-1981

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Abstract

Nanotubes (NTs) are thin, long membranous structures forming novel, yet poorly known communication pathways between various cell types. Key mechanisms controlling their growth still remained poorly understood. Since NT-forming capacity of immature and mature B cells was found largely different, we investigated how lipid composition and molecular order of the membrane affect NT-formation. Screening B cell lines with various differentiation stages revealed that NT-growth linearly correlates with membrane ganglioside levels, while it shows maximum as a function of cholesterol level. NT-growth of B lymphocytes is promoted by raftophilic phosphatidylcholine and sphingomyelin species, various glycosphingolipids, and docosahexaenoic acid-containing inner leaflet lipids, through supporting membrane curvature, as demonstrated by comparative lipidomic analysis of mature versus immature B cell membranes. Targeted modification of membrane cholesterol and sphingolipid levels altered NT-forming capacity confirming these findings, and also highlighted that the actual lipid raft number may control NT-growth via defining the number of membrane-F-actin coupling sites. Atomic force microscopic mechano-manipulation experiments further proved that mechanical properties (elasticity or bending stiffness) of B cell NTs also depend on the actual membrane lipid composition. Data presented here highlight importance of the lipid side in controlling intercellular, nanotubular, regulatory communications in the immune system.

Item Type: Article
Uncontrolled Keywords: DOMAINS; immune cells; TUNNELING NANOTUBES; IMMUNOLOGICAL SYNAPSE; Fluorescence microscopy; ATOMIC-FORCE; PLASMA-MEMBRANE; Membrane nanotubes; T-CELL-ACTIVATION; Membrane mechanics; Lipid order/fluidity; sphingolipids; Cholesterol; LIPIDOMICS; B cell membrane nanotubes
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 25 Jan 2018 08:21
Last Modified: 25 Jan 2018 08:21
URI: http://real.mtak.hu/id/eprint/73308

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