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The loops facing the active site of prolyl oligopeptidase are crucial components in substrate gating and specificity

Szeltner, Zoltán and Juhász, Tünde and Szamosi, Ilona and Rea, Dean and Fülöp, Vilmos and Módos, Károly and Polgár, László (2013) The loops facing the active site of prolyl oligopeptidase are crucial components in substrate gating and specificity. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834 (1). pp. 98-111. ISSN 1570-9639

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Abstract

Prolyl oligopeptidase (POP) has emerged as a drug target for neurological diseases. A flexible loop structure comprising loop A (res. 189-209) and loop B (res. 577-608) at the domain interface is implicated in substrate entry to the active site. Here we determined kinetic and structural properties of POP with mutations in loop A, loop B, and in two additional flexible loops (the catalytic His loop, propeller Asp/Glu loop). POP lacking loop A proved to be an inefficient enzyme, as did POP with a mutation in loop B (T590C). Both variants displayed an altered substrate preference profile, with reduced ligand binding capacity. Conversely, the T202C mutation increased the flexibility of loop A, enhancing the catalytic efficiency beyond that of the native enzyme. The T590C mutation in loop B increased the preference for shorter peptides, indicating a role in substrate gating. Loop A and the His loop are disordered in the H680A mutant crystal structure, as seen in previous bacterial POP structures, implying coordinated structural dynamics of these loops. Unlike native POP, variants with a malfunctioning loop A were not inhibited by a 17-mer peptide that may bind non-productively to an exosite involving loop A. Biophysical studies suggest a predominantly closed resting state for POP with higher flexibility at the physiological temperature. The flexible loop A, loop B and His loop system at the active site is the main regulator of substrate gating and specificity and represents a new inhibitor target. © 2012 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Bacteria (microorganisms); TEMPERATURE; protein structure; priority journal; MUTATION; MUTAGENESIS; ligand binding; KINETICS; enzyme substrate; enzyme specificity; enzyme mechanism; enzyme active site; Crystallography; crystal structure; ARTICLE; prolyl endopeptidase; X-RAY CRYSTALLOGRAPHY; Protein Engineering; prolyl oligopeptidase; enzyme structure; enzyme catalysis; Differential scanning calorimetry
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 22 Nov 2013 11:46
Last Modified: 22 Nov 2013 11:46
URI: http://real.mtak.hu/id/eprint/7451

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