Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

Sebesta, Marek and Burkovics, Péter and Juhász, Szilvia and Zhang, Sufang and Szabó, Judit E. and Haracska, Lajos (2013) Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. DNA REPAIR, 12 (9). pp. 691-698. ISSN 1568-7864

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Abstract

Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

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