Tyrosine specific sequential labeling of proteins

Cserép, G. B. and Herner, András and Wolfbeis, O. S. and Kele, Péter (2013) Tyrosine specific sequential labeling of proteins. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 23. pp. 5776-5778. ISSN 0960-894X

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We report (a) on the synthesis of a long-wavelength fluorescent coumarin containing an allyloxy acetate moiety, (b) the synthesis of two linkers containing an allyloxy acetate and an alkyne or azide function, respectively, and (c) the selective modification human serum albumin by a sequential method involving Pd(II) catalyzed modification of the phenolic side chain of tyrosine residues with an alkyne bearing linker and a subsequent azide-alkyne click reaction with an azide functionalized long-wavelength emitting coumarin dye. The method is likely to be applicable to various kinds of azido-modified fluorophores, and the Pd(II)-catalyzed modification of the tyrosines may also be used to introduce other kinds of tags. With these reagents, tyrosine specific modulation of proteins and peptides becomes possible either directly or in a sequential manner.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia
R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan
R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Depositing User: MTMT SWORD
Date Deposited: 02 Dec 2013 15:31
Last Modified: 02 Dec 2013 15:45

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