A histological and SEM study of early stages of direct somatic embryogenesis in leaves of sundew Drosera spathulata Labill.

Bobák, M. and Šamaj, J. and Blehová, A. and Ovečka, M. and Hlavačka, A. and Illéš, P. and Kutarňová, Z. (2006) A histological and SEM study of early stages of direct somatic embryogenesis in leaves of sundew Drosera spathulata Labill. Acta Botanica Hungarica, 48 (1-2). pp. 29-38. ISSN 0236-6495

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Abstract

The possibility of plant regeneration via direct somatic embryogenesis from leaf tissue of Drosera spathulata Labill. has been studied, using MS medium supplemented with various concentration of NAA and BAP. Scanning electron microscopy investigations in combination with histological analysis verified direct somatic embryogenesis by formation of globular, torpedo-shaped, heart-shaped and cotyledonary embryo like structures. Optimal somatic embryo formation occurred when leaves were pre-treated with low concentration (0.005 mg l^-1) of 2,4-D within 6 h. Prolonged influence of 2,4-D to 24-48 h led to the expression of morphological and developmental abnormalities.

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