Increased chilling tolerance and altered protein patterns in tomato seedlings following hardening of seeds or system in application

Shukry, W. and El-Otaby, L. (2011) Increased chilling tolerance and altered protein patterns in tomato seedlings following hardening of seeds or system in application. Acta Botanica Hungarica, 53 (3-4). pp. 389-408. ISSN 0236-6495

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Abstract

Plants have evolved complex mechanisms to perceive environmental cues and develop appropriate and coordinated responses to abiotic and biotic stresses. Considerable progress has been made towards a better understanding of the molecular mechanisms of plant response to a single stress. However, the existence of cross-tolerance to different stressors has proved to have great relevance in the control and regulation of organismal adaptation. In this study, we showed that cold pre-treatment (at 15, 10, 5 and 0 °C) enhanced chilling stress in two cultivars of tomato plants (Lycopersicon esculentum cv. ‘Tres Cantos’ and ‘Roma’), as indicated by de novo synthesis of stress proteins in the cold-treated plants than the control ones. The cold-treated ‘Roma’ seedlings were more resistant to chilling stress than ‘Cantos’. Active synthesis of 43 kDa heat shock proteins and some low molecular weight proteins took place after chilling stress in both cultivars. Seeds pre-treatment with systemin at three concentrations (10−6, 10−2 and 100μM) or spraying the seedlings of two plants with the same concentrations leads to stressful polypeptides were detected as 16 kDa which is one from low molecular weight proteins were referred as vegetative storage proteins (VSPs). In ‘Tres Cantos’, the spraying of plants was more effective than the pre-soaking, while in ‘Roma’, the pre-soaking with systemin is more effective at low and moderate concentrations (10−6 and 10−2 μM), meanwhile, the spraying with systemin is more effective at high concentration (100 μM).

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