Inhibition of the formation of amyloid-like fibrils using herbal extracts

Kotormán, Márta and Kelemen, Zita and Kasi, Phanindra Babu and Nemcsók, János (2018) Inhibition of the formation of amyloid-like fibrils using herbal extracts. Acta Biologica Hungarica, 69 (2). pp. 125-134. ISSN 0236-5383


Download (780kB) | Preview


We tested the amyloid fibril formation inhibitory effect of seven teas diluted in 55% ethanol at pH 7.0 at a protein concentration of 0.15 mg/ml α-chymotrypsin. In the experiments we investigated the formation and inhibition of amyloid fibrils by turbidity measurements, aggregation kinetics experiments and Congo red binding assay. The results suggest that the different teas effectively inhibit the formation of amyloidlike fibrils. The two most potent inhibitors were peppermint and melilot, extracts which almost completely inhibited the formation of aggregates in 5-fold dilution. The inhibitory effect on the aggregation formation of melilot and peppermint extracts was concentration dependant. The extent of inhibition was found to be proportional with the total concentration of phenolic compounds.

Item Type: Article
Additional Information: MTA KFB támogatási szerződés alapján archiválva
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Violetta Baliga
Date Deposited: 02 Jul 2018 14:11
Last Modified: 30 Jun 2019 23:16

Actions (login required)

Edit Item Edit Item