Effects of the venom of the brown bullhead catfish (Ameiurus nebulosus) on isolated smooth muscles

Barthó, Loránd and Sándor, Zsolt and Bencsik, Timea (2018) Effects of the venom of the brown bullhead catfish (Ameiurus nebulosus) on isolated smooth muscles. Acta Biologica Hungarica, 69 (2). pp. 135-143. ISSN 0236-5383


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Aqueous extract of the spines of the brown bullhead catfish (Ameiurus nebulosus Lesueur, 1819) caused contraction of the guinea-pig small intestine in vitro, a widely-used preparation in pharmacology. The action is dependent on extracellular Ca<sup>2+</sup>, and probably takes place on the smooth muscle cells. Mouse gastrointestinal preparations were also contracted by the extract. Stings by the spines of this fish species causes a painful sensation in man. We tested the effect of an extract of spines in isolated organ experiments on innervated smooth muscle preparations. In the guinea-pig ileum, the response to the extract was abolished by the Ca<sup>2+</sup>-channel blocker nifedipine, but only slightly reduced by atropine (a muscarine receptor antagonist) or tetrodotoxin (TTX; a blocker axonal conduction) or antagonists for P<sub>2X</sub> purinoceptors. Blocking of serotonin or histamine H<sub>1</sub> receptors, tachykinin NK<sub>1</sub> receptors, functional impairment of capsaicin-sensitive sensory nerve endings or inhibition of cyclo-oxygenases failed to influence the contractile effect of the extract. No inhibitory action of the extract was detected on the ileum subject to electrical motor nerve stimulation.

Item Type: Article
Additional Information: MTA KFB támogatási szerződés alapján archiválva
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Violetta Baliga
Date Deposited: 02 Jul 2018 14:15
Last Modified: 30 Jun 2019 23:16

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