Performance of immobilized bacterial alpha-amylases in methyltriethoxsilane / tetraetoxysilane sol-gel matrices

VLAD-OROS, BEATRICE and Oniga, O. and Dudás, Zoltán Imre (2007) Performance of immobilized bacterial alpha-amylases in methyltriethoxsilane / tetraetoxysilane sol-gel matrices. ANNALS OF WEST UNIVERSITY OF TIMISOARA-SERIES CHEMISTRY, 16 (2). pp. 261-266. ISSN 1224-9513

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Abstract

The large number of studies related to the field of biomolecules encapsulation in sol-gel hosts clearly indicates that this approach can be considered as a powerful alternative to traditional encapsulation procedures involving biopolymer hosts. In this study, α-amylase was immobilized, by using the sol-gel technique, in silica particles obtained from hydrolysis and polycondensation of tetraethoxysilane (TEOS) and a mixture of methyltriethoxysilane (MTES) and tetraethoxysilane. The influence of the pH and temperature of free and immobilized α-amylase were compared. It was shown that the relative activities of immobilized enzymes are higher than those of free enzymes over broader pH and temperature ranges. The Michaelis constant and the maximum rate of starch hydrolysis reaction were calculated by fitting the experimental data to the Michaelis-Menten equation. It was found that KM and Vmax values of the immobilized enzyme were smaller than those of the free enzyme.

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