Sensitive method for glycosaminoglycan analysis of tissue sections

Turiák, Lilla and Tóth, Gábor and Ozohanics, Olivér and Rokobné Révész, Ágnes and Ács, András and Vékey, Károly and Drahos, László (2018) Sensitive method for glycosaminoglycan analysis of tissue sections. JOURNAL OF CHROMATOGRAPHY A, 1544. pp. 41-48. ISSN 0021-9673

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Abstract

A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC-MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1fmol; quantitation limits 10fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10mug).

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