A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces

Tátrai, Péter and Sági, Bernadett and Szigeti, Anna and Szepesi, Áron and Szabó, Ildikó and Bősze, Szilvia and Kristóf, Zoltán and Markó, Károly and Szakács, Gergely and Urbán, István and Mező, Gábor and Német, Katalin (2013) A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 24 (2). pp. 479-488. ISSN 0957-4530

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Abstract

Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Ser i -DL-Ala m ) polymer functionalized with cyclic arginyl-glycyl-aspar- tate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio- Oss bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.

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