Knorr, Gergely and Kozma, Eszter and Herner, András and Lemke, E. A. and Kele, Péter (2016) New Red-Emitting Tetrazine-Phenoxazine Fluorogenic Labels for Live-Cell Intracellular Bioorthogonal Labeling Schemes. CHEMISTRY-A EUROPEAN JOURNAL, 22 (26). pp. 8972-8979. ISSN 0947-6539
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Abstract
The synthesis of a set of tetrazine-bearing fluorogenic dyes suitable for intracellular labeling of proteins in live cells is presented. The red excitability and emission properties ensure minimal autofluorescence, while through-bond energy-transfer-based fluorogenicity reduces nonspecific background fluorescence of unreacted dyes. The tetrazine motif efficiently quenches fluorescence of the phenoxazine core, which can be selectively turned on chemically upon bioorthogonal inverse-electron-demand Diels-Alder reaction with proteins modified genetically with strained trans-cyclooctenes. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Item Type: | Article |
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Uncontrolled Keywords: | PROTEINS; FLUORESCENCE; biosynthesis; fluorescent probes; imaging agents; Energy transfer; Imaging agent; autofluorescence; Labeling scheme; Emission properties; Inverse-electron demand diels-alder reactions; Bond energies; Dyes/pigments; |
Subjects: | Q Science / természettudomány > QD Chemistry / kémia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 17 Jan 2019 13:11 |
Last Modified: | 17 Jan 2019 13:11 |
URI: | http://real.mtak.hu/id/eprint/90123 |
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