The effect of increased NaCl intake on rat brain endogenous mu-opioid receptor signalling

Dadam, Florencia and Zádor, Ferenc and Caeiro, Ximena and Szűcs, Edina and Erdei, Anna and Samavati, Reza and Gáspár, Róbert and Borsodi, Anna and Vivas, Laura (2018) The effect of increased NaCl intake on rat brain endogenous mu-opioid receptor signalling. JOURNAL OF NEUROENDOCRINOLOGY, 30 (4). ISSN 0953-8194


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Numerous studies demonstrate the significant role of central -endorphin and its receptor, the mu-opioid receptor (MOR), in sodium intake regulation. The present study aimed to investigate the possible relationship between chronic high-NaCl intake and brain endogenous MOR functioning. We examined whether short-term (4 days) obligatory salt intake (2% NaCl solution) in rats induces changes in MOR mRNA expression, G-protein activity and MOR binding capacity in brain regions involved in salt intake regulation. Plasma osmolality and electrolyte concentrations after sodium overload and the initial and final body weight of the animals were also examined. After 4 days of obligatory hypertonic sodium chloride intake, there was clearly no difference in MOR mRNA expression and G-protein activity in the median preoptic nucleus (MnPO). In the brainstem, MOR binding capacity also remained unaltered, although the maximal efficacy of MOR G-protein significantly increased. Finally, no significant alterations were observed in plasma osmolality and electrolyte concentrations. Interestingly, animals that received sodium gained significantly less weight than control animals. In conclusion, we found no significant alterations in the MnPO and brainstem in the number of available cell surface MORs or de novo syntheses of MOR after hypertonic sodium intake. The increased MOR G-protein activity following acute sodium overconsumption may participate in the maintenance of normal blood pressure levels and/or in enhancing sodium taste aversion and sodium overload-induced anorexia.

Item Type: Article
Uncontrolled Keywords: ACTIVATION; GENE-EXPRESSION; SOLITARY TRACT; AGONIST; BETA-ENDORPHIN; AMYGDALA; brainstem; Appetite; salt intake; lateral parabrachial nucleus; HYPERTONIC SODIUM-INTAKE; sodium ingestion; G-protein activation; mu-opioid receptor signalling;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Depositing User: MTMT SWORD
Date Deposited: 31 Jan 2019 06:36
Last Modified: 28 Feb 2019 00:15

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