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Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas

Jia, Xinkai and Gábris, Fanni and Jacobsen, Oli and Bedics, Gábor and Botz, Bálint and Helyes, Zsuzsanna and Kellermayer, Zoltán and Vojkovics, Dóra and Berta, Gergely and Nagy, Nándor and Jakus, Zoltán and Balogh, Péter (2020) Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas. JOURNAL OF IMMUNOLOGY, 204 (1). pp. 23-36. ISSN 0022-1767

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Abstract

The cellular homeostasis of lymphoid tissues is determined by the continuous interactions of mobile hematopoietic cells within specialized microenvironments created by sessile stromal cells. In contrast to the lymph nodes and mucosal lymphoid tissues with well-defined entry and exit routes, the movement of leukocytes in the peritoneal cavity is largely unknown. In this study, we report that, in addition to the omental milky spots and fat-associated lymphoid clusters, in mice, the serous surface of the mesenteric adipose streaks contains lymphocyte-rich organoids comprised of a highly compacted leaf-like part connected to the adipose tissue that can also efficiently bind B cells and high-grade B cell lymphoma (diffuse large B cell lymphoma) cells. Denoted as foliate lymphoid aggregates (FLAgs), these structures show incomplete T/B segregation and a partially differentiated stromal architecture. LYVE-1-positive macrophages covering FLAgs efficiently bind i.p. injected normal B cells as well as different types of diffuse large B cell lymphoma cells. Within FLAgs, the lymphocytes compartmentalize according to their chemokine receptor pattern and subsequently migrate toward the mesenteric lymph nodes via the mesenteric lymphatic capillaries. The blood supply of FLAgs includes short vascular segments displaying peripheral lymph node addressin, and the extravasation of lymphocytes to the omental and mesenteric adipose tissues is partly mediated by L-selectin. The appearance of i.p. injected cells in mesenteric lymph nodes suggests that the mesentery-associated lymphatics may also collect leukocytes from the fat-associated lymphoid clusters and FLAgs, thus combining the mucosal and serous exit of mobile leukocytes and increasing the range of drainage sites for the peritoneal expansion of lymphoid malignancies.

Item Type: Article
Additional Information: Funding Agency and Grant Number: Hungarian Science Foundation National Research, Development and Innovation Office [128322, GINOP-2.3.2-15-2016-00022]; Hungarian Science Foundation NKFI Grant [124740]; New National Excellence Program of the Ministry of Human Capacities of Hungary [UNKP-17-4-I-PTE-83]; Faculty of Medicine, University of Pecs; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-19-4-P-PTE-458]; University of Pe'cs projects [GINOP-2.3.2-15-2016-00048, EFOP-3.6.1-16-2016-00004]; University of Szeged project [EFOP-3.6.2-16-2017-00006] Funding text: This work was supported by Hungarian Science Foundation National Research, Development and Innovation Office Grants 128322 (P.B.) and GINOP-2.3.2-15-2016-00022. N.N. was supported by Hungarian Science Foundation NKFI Grant 124740. Z.K. was supported by the UNKP-17-4-I-PTE-83 New National Excellence Program of the Ministry of Human Capacities of Hungary and the postdoctoral research grant of the Faculty of Medicine, University of Pecs. B.B. was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences and the UNKP-19-4-P-PTE-458 New National Excellence Program of the Ministry for Innovation and Technology. Z.H. was supported by University of Pe ' cs projects GINOP-2.3.2-15-2016-00048 (Stay Alive) and EFOP-3.6.1-16-2016-00004 and University of Szeged project EFOP-3.6.2-16-2017-00006 (Live Longer).
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia > QR180 Immunology / immunológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 26 Sep 2020 06:23
Last Modified: 26 Sep 2020 06:23
URI: http://real.mtak.hu/id/eprint/114637

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