Tóth, Eszter and Ozohanics, Olivér and Bobály, Balázs and Gömöry, Ágnes and Jekő, Anita and Drahos, László and Vékey, Károly (2014) HPLC enrichment/isolation of proteins for post-translational modification studies from complex mixtures. Journal of Pharmaceutical and Biomedical Analysis, 98. pp. 393-400. ISSN 0731-7085
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Abstract
The paper describes a macroporous RP-HPLC method for separation and isolation/enrichment of proteins from complex mixtures. The method is robust and efficient; using 2.1 or 4.6 mm diameter columns provides sufficient material for subsequent proteomic analysis. The main advantage of the method is that most protein variants are isolated in the same fraction, as separation is not based on differences in isoelectric point. This is highly advantageous for studying complex mixtures and post-translational modifications. Examples related to glycosylation analysis are discussed in detail.
| Item Type: | Article |
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| Uncontrolled Keywords: | Mass spectrometry; HPLC; sample preparation; proteins; glycosylation |
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
| Depositing User: | Eszter Tóth |
| Date Deposited: | 26 Jan 2015 10:19 |
| Last Modified: | 26 Jan 2015 10:19 |
| URI: | http://real.mtak.hu/id/eprint/20940 |
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