Calibration-less sizing and quantitation of polymeric nanoparticles and viruses with quartz nanopipettes

Terejánszky, Péter and Makra, István and Fürjes, Péter and Gyurcsányi, Ervin Róbert (2014) Calibration-less sizing and quantitation of polymeric nanoparticles and viruses with quartz nanopipettes. ANALYTICAL CHEMISTRY, 86 (10). pp. 4688-4697. ISSN 0003-2700

Calibration-less sizing and quantification.pdf

Download (1MB) | Preview


The feasibility of using quartz nanopipets as simple and cost-effective Coulter counters for calibration-less quantitation and sizing of nanoparticles by resistive pulsing sensing (RPS) was investigated. A refined theory was implemented to calculate the size distribution of nanoparticles based on the amplitude of resistive pulses caused by their translocation through nanopipets of known geometry. The RPS provided diameters of monodisperse latex nanoparticles agreed within the experimental error with those measured by using scanning electron microscopy (SEM), dynamic light scattering (DLS), and nanoparticle tracking analysis (NTA). The nanopipet-based counter, by detecting individual nanoparticles, could resolve with similar resolution as SEM mixtures of monodisperse nanoparticles having partially overlapping size distributions, which could not be discriminated by DLS or NTA. Furthermore, by calculating the hydrodynamic resistance of the nanopipets and consequently the volume flow through the tip enabled for the first time the calibration-less determination of nanoparticle concentrations with nanopipets. The calibration-less methodology is applied to sizing and quantitation of inactivated poliovirus of ∼26 nm diameter, which is the smallest size spherical shape virus ever measured by resistive pulse sensing.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Q Science / természettudomány > QD Chemistry / kémia
Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
Depositing User: Berényi Szilvia
Date Deposited: 12 Feb 2015 09:33
Last Modified: 12 Feb 2015 09:33

Actions (login required)

Edit Item Edit Item