Examinations of allergenic proteins coding genes of some domestic apple cultivars

Szabó, E. and Gelencsér, É. and Kovács, E. and Jánosi, A. and Takács, K. and Kiss, E. (2012) Examinations of allergenic proteins coding genes of some domestic apple cultivars. Acta Alimentaria, 41 (1). pp. 8-18. ISSN 0139-3006

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Abstract

In our research we studied the occurrence of the main apple allergen coding gene-families (Mal d 1, Mal d 2, Mal d 3, Mal d 4) in 16 different and most preferably consumed apple varieties. After the DNA isolation by Wizard method the simple PCR reaction was used to examine the apple allergen-coding genes. To identify the presence of the four allergenic protein-coding genes two primer pairs were chosen. The presence of these allergens in most apple varieties could be confirmed. According to our results two varieties — Jonathan and Granny Smith — were found to contain the lowest amount of the coding genes of the allergenic apple proteins studied by us. Besides this, polymorph pattern was obtained by the use of Mal d 1 primer, which may lead to determine apple varieties with small amount of Mal d 1 allergens.The confirmation study of the presence of potential apple allergens by RNA and protein techniques is our plan in the near future.

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