Behaviour of carp proteins and corn proteins in three-phase partitioning

Szamos, J. (2006) Behaviour of carp proteins and corn proteins in three-phase partitioning. Acta Alimentaria, 35 (1). pp. 109-116. ISSN 0139-3006

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Abstract

In three-phase partitioning (TPP) ammonium sulphate and 2-methyl-2-propanol (tert-butanol) are used for separation of proteins by collecting and concentrating them into the middle layer or third phase of the system. During the last two decades, TPP has been primarily applied to enzyme purification at laboratory scale, however, its analytical type application for investigating meat has also been reported. Here, an experimental set of TPP feasible to analytical scale experiments is described to study partitioning behaviour of carp and corn proteins. In partitioning experiments, corn proteins extractable with borate buffer, and sarcoplasmic proteins of carp were investigated. In the pH-range of 4.8-7.9, composition of partitioned corn proteins showed independence of pH as it was indicated by nonequilibrium pH gradient electrophoresis (NEpHGE) patterns. In case of carp, the alterations of protein composition in the pH-range of 3.7-8.0 unambiguously indicated the pH-dependent partitioning of carp proteins. The analytical scale method, based on three-phase partitioning is a new approach to investigate food proteins.

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