Kurunczi, Sándor and Hainard, Alexandre and Juhász, Krisztina and Patkó, Dániel and Orgovan, Norbert and Truck, Natacha and Sanchez, Jean-Charles and Horváth, Róbert (2013) Polyethylene imine-based receptor immobilization for label free bioassays. SENSORS AND ACTUATORS B-CHEMICAL, 181. pp. 71-76. ISSN 0925-4005
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Abstract
Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Room temperature immobilization Polyethylene imine (PEI) Label free sensor Neutravidin C-reactive protein (CRP) OWLS |
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában T Technology / alkalmazott, műszaki tudományok > TD Environmental technology. Sanitary engineering / környezetvédelem, hulladékkezelés, egészségügyi mérnöki technika (ivóvízellátási és szennyvízkezelési technika) T Technology / alkalmazott, műszaki tudományok > TP Chemical technology / vegyipar, vegyészeti technológia |
| Depositing User: | Andrea Bolgár |
| Date Deposited: | 25 Apr 2013 14:37 |
| Last Modified: | 04 Apr 2023 12:28 |
| URI: | http://real.mtak.hu/id/eprint/4919 |
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