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Polyethylene imine-based receptor immobilization for label free bioassays

Kurunczi, Sándor and Hainard, Alexandre and Juhász, Krisztina and Patkó, Dániel and Orgovan, Norbert and Truck, Natacha and Sanchez, Jean-Charles and Horváth, Róbert (2013) Polyethylene imine-based receptor immobilization for label free bioassays. SENSORS AND ACTUATORS B-CHEMICAL, 181. pp. 71-76. ISSN 0925-4005

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Abstract

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor.

Item Type: Article
Uncontrolled Keywords: Room temperature immobilization Polyethylene imine (PEI) Label free sensor Neutravidin C-reactive protein (CRP) OWLS
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
T Technology / alkalmazott, műszaki tudományok > TD Environmental technology. Sanitary engineering / környezetvédelem, hulladékkezelés, egészségügyi mérnöki technika (ivóvízellátási és szennyvízkezelési technika)
T Technology / alkalmazott, műszaki tudományok > TP Chemical technology / vegyipar, vegyészeti technológia
Depositing User: Andrea Bolgár
Date Deposited: 25 Apr 2013 14:37
Last Modified: 25 Apr 2013 14:37
URI: http://real.mtak.hu/id/eprint/4919

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