Anticancer effects of selenium compounds on human colonic carcinoma cells

Glavaš-Obrovac, Lj. and Klapec, T. and Karner, I. and Mandić, M. L. (2000) Anticancer effects of selenium compounds on human colonic carcinoma cells. Acta Alimentaria, 29 (3). pp. 295-306. ISSN 0139-3006

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Abstract

Studies performed so far on different human carcinoma cell lines, as well as numerous case-control and epidemiological studies have given proof to the protective effects of selenium against cancer. However, the anticancer properties of selenium are site-specific. The aim of this work was to evaluate the cytotoxic effect of selenium against CaCo2 human colon carcinoma cells, and SW620 lymph node metastasis of colon carcinoma cell line. Three selenium compounds, seleno-DL-cystine (SeC), seleno-L-methionine (SeM) and sodium selenite were used. Initial number of cells was 210 4 and the cells were incubated for 72 h with the aforementioned Se compounds at 10, 100 and 1000 µmol Se concentrations. Cytotoxicity was measured by the MTT cell survival assay. In the present study, decreased viabilities of both CaCo2 and SW620 cells were established following the treatment with selenite, SeC, and SeM. At 10 µmol Se levels all three chemical forms exerted a more or less anticipated cytotoxic effect with viability decreases ranging from 22 to 37%. However, the other two levels of 100 and 1000 µmol Se did not exhibit an expected proportional rise in cytotoxic effect compared to 10 µmol, which warrants further research on the reasons for increased resistance of these cells. Cell morphology also indicates that investigated Se forms induced apoptotic cell death in both cell lines. The results confirm the applicability of Se in the prevention and treatment of the investigated cancer sites.

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