In depth proteomic analysis of prostate cancer biopsies

Tóth, Gábor and Ozohanics, Oliver and Ács, András and Révész, Ágnes and Vékey, Károly and Drahos, László and Turiák, Lilla (2018) In depth proteomic analysis of prostate cancer biopsies. In: MSACL-Annual Congress in Clinical Mass Spectrometry, 2018. szeptember 11-13, Salzburg, Ausztria.

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Abstract

Cancer research is among the most studied areas of science and prostate cancer (PCa) is one of the most common types of cancer among men. In this work we describe a detailed proteomics analysis of PCa tissue microarrays (TMAs) with our novel methodology. It is based on surface proteolytic digestion and proved to be capable of quantifying over 500 proteins from a single 1.5 mm diameter TMA core. We have compared the protein composition of tissues with various grades and stages of cancer. Samples from healthy and cancerous tissues were clearly distinguished and a good correlation with cancer grade was found. A well balanced study was carried out and over 100 proteins showed statistically significant abundance changes between various groups. During STRING evaluation up-regulation eg. in KEGG ribosome pathway and mRNA splicing could be observed.

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