REAL

Distinguishing core and antenna fucosylated glycopeptides based on low energy tandem mass spectra

Ács, András and Ozohanics, Oliver and Vékey, Károly and Drahos, László and Turiák, Lilla (2018) Distinguishing core and antenna fucosylated glycopeptides based on low energy tandem mass spectra. ANALYTICAL CHEMISTRY, 90 (21). pp. 12776-12782. ISSN 0003-2700

[img]
Preview
Text
Manuscript File_Anal_Chem.pdf

Download (746kB) | Preview

Abstract

A straightforward approach has been developed to distinguish core and antenna fucosylation in glycopeptides. The method does not require derivatization, and can be easily adapted into a proteomics workflow. The key aspect is to use low collision energy CID (on a QTOF type instrument) when only single step fragmentation processes occur. Low collision energy should show the precursor ion as the largest peak in the spectrum; the survival yield should be ideally over 50%; and this is obtained at a collision energy ca. 30% of that typically used for proteomics. In such a case interfering processes like fucose migration or consecutive reactions are minimized. Core and antenna fucosylation can be discriminated using various ion abundance ratios. Low energy CID spectra are very “clean” (no chemical noise), and the ions used for locating the fucose are among the major peaks; making the method well suited for analytical work. Monitoring the change in the proportion of core and antenna fucosylation at the same glycosylation site is also feasible.

Item Type: Article
Uncontrolled Keywords: mass spectrometry, glycopeptide, fragmentation, CID, energy-dependent, fucosylation
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
Depositing User: Ágnes Gömöry
Date Deposited: 31 Jan 2019 09:33
Last Modified: 31 Jan 2019 09:34
URI: http://real.mtak.hu/id/eprint/90874

Actions (login required)

Edit Item Edit Item