Analysis of propagation of slow rhythmic activity induced in ex vivo rat brain slices

Balogh, Veronika and Szádeczky-Kardoss, Katalin and Varró, Petra and Világi, Ildikó and Borbély, Sándor (2019) Analysis of propagation of slow rhythmic activity induced in ex vivo rat brain slices. Brain Connectivity. ISSN 2158-0022

Text 10.1089@brain.2018.0650.pdf - Published Version Restricted to Registered users only Download (4MB)

Abstract

Slow wave oscillation is a synchronous oscillatory mechanism that is a characteristic wave type of the cerebral cortex during physiological deep sleep or anesthesia. It may play an important role in cortical analysis of sensory input. Our goal was (1) to develop optimal conditions for the induction of this slow rhythmic activity in adult rat cortical slices, (2) to identify connections through which the activity propagates between coupled cortical re- gions, and (3) to study the pattern of horizontal and vertical flow of activity developed spontaneously in cortical slices. Experiments were performed on intact or differently incised rat cortical slices. According to our results, spontaneous cortical activity develops reliably in slightly modified artificial cerebrospinal fluid, first in the ento- rhinal cortical region of horizontally cut slices and then it spreads directly to the perirhinal (PRh) cortex. The activity readily generated in layer 2/3 of the entorhinal cortex then quickly spreads vertically to upper layer 2–3 in the same area and to the neighboring regions, that is, to the PRh cortex. Synchronization of activity in neighboring cortical areas occurs through both callosal connections and layer 2–3 intrinsic network, which are important in the propagation of spontaneous, inherent cortical slow wave activity.

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