REAL

Environmental pH and a Glu364 to Gln mutation in the chlorophyll-binding CP47 protein affect redox-active TyrD and charge recombination in Photosystem II

Morris, Jaz N. and Kovács, Sándor and Vass, Imre and Summerfield, Tina C. and Eaton-Rye, Julian J. (2019) Environmental pH and a Glu364 to Gln mutation in the chlorophyll-binding CP47 protein affect redox-active TyrD and charge recombination in Photosystem II. FEBS LETTERS, 593 (2). pp. 163-174. ISSN 0014-5793

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Abstract

In Photosystem II, loop E of the chlorophyll-binding CP47 protein is located near a redox-active tyrosine, Y-D, forming a symmetrical analog to loop E in CP43, which provides a ligand to the oxygen-evolving complex (OEC). A Glu364 to Gln substitution in CP47, near Y-D, does not affect growth in the cyanobacterium Synechocystis sp. PCC 6803; however, deletion of the extrinsic protein PsbV in this mutant leads to a strain displaying a pH-sensitive phenotype. Using thermoluminescence, chlorophyll fluorescence, and flash-induced oxygen evolution analyses, we demonstrate that Glu364 influences the stability of Y-D and the redox state of the OEC, and highlight the effects of external pH on photosynthetic electron transfer in intact cyanobacterial cells.

Item Type: Article
Additional Information: Funding Agency and Grant Number: Senior Smeaton Prize in Experimental Science at the University of Otago; [NKFI-H (NN-110960)]; [GINOP-2.3.2-15-2016-00001] Funding text: We thank Dr Simon A. Jackson for assistance with use of the Joliot electrode, and Laszlo Sass for assistance with the operation of TL-measuring equipment and the analysis of the O2 flash patterns. JNM was supported by the Senior Smeaton Prize in Experimental Science at the University of Otago. The laboratory of IV is supported by grant number NKFI-H (NN-110960) and GINOP-2.3.2-15-2016-00001.
Uncontrolled Keywords: CRYSTAL-STRUCTURE; OXIDATION; STRAIN; CYANOBACTERIA; Biophysics; PHOTOSYSTEM II; DIRECTED MUTAGENESIS; OXYGEN-EVOLVING COMPLEX; EXTRINSIC PROTEINS; Synechocystis; TYROSINE-D; Biochemistry & Molecular Biology; CP47; Synechocystis sp. PCC 6803; TyrD; PCC 6803; PHOTOAUTOTROPHIC GROWTH;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 23 Nov 2019 13:16
Last Modified: 23 Nov 2019 13:16
URI: http://real.mtak.hu/id/eprint/103642

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