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“Fishing and hunting”– Selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media

Sánta-Bell, Evelin and Molnár, Zsófia Klára and Varga, Andrea and Nagy, Flóra and Hornyánszky, Gábor and Paizs, Csaba and Balogh Weiser, Diána and Poppe, László (2019) “Fishing and hunting”– Selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media. MOLECULES, 24 (22). ISSN 1420-3049

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Abstract

This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD04 Organic chemistry / szerves kémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 29 Nov 2019 15:06
Last Modified: 29 Nov 2019 15:06
URI: http://real.mtak.hu/id/eprint/103991

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