Berberine alkaloid as a sensitive fluorescent probe for bile salt aggregates

Megyesi, Mónika and Biczók, László (2007) Berberine alkaloid as a sensitive fluorescent probe for bile salt aggregates. JOURNAL OF PHYSICAL CHEMISTRY B - CONDENSED MATTER MATERIALS SURFACES INTERFACES AND BIOPHYSICAL, 111 (20). pp. 5635-5639. ISSN 1520-6106

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Abstract

Effect of sodium cholate (NaC) bile salt on the absorption and fluorescence properties of berberine cation was studied in aqueous solution and water-cosolvent mixtures. The alteration of the fluorescent behavior with increasing NaC concentration showed an entirely different trend from that found previously in the presence of sodium dodecylsulfate. Binding to bile salt agglomerates led to significant fluorescence intensity enhancement, and the fluorescence lifetime of berberine proved to be highly sensitive to the structure and size of the aggregates. The dual exponential decay kinetics above 10 mM NaC concentration showed that the probe resided in two totally different binding sites. At 2-10 mM NaC concentrations, only primary aggregates were detected. The aggregate disrupting power of cosolvents decreased in the series of dimethylformamide, acetonitrile, formamide, and methanol. These compounds enhanced the water accessibility of berberine bound to aggregates and diminished the number of secondary aggregates.

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