Use of H. vulgare EST Markers, GISH and C-banding to Study Bread Wheat – H. marinum subsp. gussoneanum (2n = 28) Introgression Lines

Trubacheeva, N.V. and Badaeva, E.D. and Osadchaya, T.S. and Pershina, L.A. (2019) Use of H. vulgare EST Markers, GISH and C-banding to Study Bread Wheat – H. marinum subsp. gussoneanum (2n = 28) Introgression Lines. Cereal Research Communications, 47 (4). pp. 593-603. ISSN 0133-3720

Preview

Text 0806.47.2019.37.pdf Download (306kB) | Preview

Abstract

Wild barley, Hordeum marinum subsp. gussoneanum (2n = 28) is a valuable source of genes that determine resistance to abiotic stresses. These resistance traits might be transferred to wheat due to the crossability of wild barley with bread wheat. The availability of reliable and rapid methods for the identification of H. marinum subsp. gussoneanum chromatin in a wheat background would facilitate the development of introgression wheat genotypes. For this purpose, we evaluated the applicability of eighty-seven H. vulgare EST markers for studying bread wheat – H. marinum subsp. gussoneanum substitution and addition lines. Of all of the markers studied, forty-three (49%) were amplified in H. marinum ssp. gussoneanum and wheat introgression lines. The identification of wild barley chromosomes using EST markers confirmed the GISH and C-banding data. Thus, it was established that the H. vulgare EST markers can be successfully used to identify the chromosomes of the H. marinum subsp. gussoneanum in introgression lines of wheat.

Actions (login required)

Edit Item