Dextran-based Hydrogel Layers for Biosensors

Saftics, András and Türk, Barbara and Sulyok, Attila and Nagy, Norbert and Agócs, Emil and Kalas, Benjamin and Petrik, Péter and Fried, Miklós and Nguyen Quoc, Khánh and Kamarás, Katalin and Székács, Inna and Horváth, Róbert and Kurunczi, Sándor (2020) Dextran-based Hydrogel Layers for Biosensors. In: Nanobiomaterial Engineering. Springer Singapore, Singapore, pp. 139-164. ISBN 9789813298408; 9789813298392

Text Nanobiomaterial_Saftics.pdf Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Biofunctional coatings are key elements of biosensors regulating interactions between the sensing surface and analytes as well as matrix components of the sample. These coatings can improve sensing capabilities both by amplifying the target signal and attenuating interfering signals originating from surface fouling (non-specific binding). Considering the tested materials so far, hydrogel-based layers have been verified to be among the most effective layers in improving biochip performance. The polysaccharide dextran can be efficiently used to form hydrogel layers displaying extended three-dimensional structure on biosensor surfaces. Owing to their high water content and flexible structure, dextran coatings present advanced antifouling abilities, which can be exploited in classic bioanalytical measurements as well as in the development of cell-on-a-chip type biosensors. However, in spite of the numerous applications, the deep characterization of dextran layers has been missing from the literature. This phenomenon can be attributed to the challenging analysis of few nanometer-thick layers with high water content. The lack of available data is more pronounced regarding the layer behaviors under aqueous conditions. In this chapter we present various surface analytical methods (including biosensor-type techniques) suitable for the complex characterization of hydrogel coatings whose thickness ranges from few to several ten nanometers. As a case study, we focus on the analysis of carboxymethyl dextran (CMD) layers developed for waveguide-based label-free optical biosensor applications. Examination methodologies both under dry and aqueous conditions as well as testing of antifouling abilities are also presented.

Actions (login required)

Edit Item