Jeney, Viktória and Komódi, E. and Nagy, E. and Zarjou, A. and Vercellotti, G.M. and Balla, György and Balla, József (2009) Supression of hemin-mediated oxidation of low-density lipoprotein and subsequent endothelial reactions by hydrogen sulfide (H2S). FREE RADICAL BIOLOGY AND MEDICINE, 46 (5). pp. 616-623. ISSN 0891-5849
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Abstract
Heme-mediated oxidative modification of low-density lipoprotein (LDL) plays a crucial role in early atherogenesis. It has been shown that hydrogen sulfide (H2S) produced by vascular smooth muscle cells is present in plasma at a concentration of about 50 mu mol/L. H2S is a strong reductant which can react with reactive oxygen species like superoxide anion and hydrogen peroxide. The current study investigated the effect of H2S on hemin-mediated oxidation of LDL and oxidized LDL (oxLDL)-induced endothelial reactions. H2S dose dependently delayed the accumulation of lipid peroxidation products-conjugated dienes, lipid hydroperoxides (LOOH), and thiobarbituric acid reactive substances-during hemin-mediated oxidation. Moreover, H2S decreased the LOOH content of both oxidized LDL and lipid extracts derived from soft atherosclerotic plaque, which was accompanied by reduced cytotoxicity. OxLDL-mediated induction of the oxidative stress responsive gene, heme oxygenase-1, was also abolished by H2S. Finally we have shown that H2S can directly protect endothelium against hydrogen peroxide and oxLDL-mediated endothelial cytotoxicity. These results demonstrate novel functions of H2S in preventing hemin-mediated oxidative modification of LDL, and consequent deleterious effects, suggesting a possible antiatherogenic action of H2S. (C) 2008 Elsevier Inc. All rights reserved.
Item Type: | Article |
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Uncontrolled Keywords: | Hydrogen sulfide Low-density lipoprotein Lipid hydroperoxide Hemin Heme oxygenase-1 |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia R Medicine / orvostudomány > RC Internal medicine / belgyógyászat R Medicine / orvostudomány > RJ Pediatrics / gyermekgyógyászat |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 30 May 2014 07:50 |
Last Modified: | 30 May 2014 07:50 |
URI: | http://real.mtak.hu/id/eprint/12932 |
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