Protective effects of PACAP against oxidative stress in cochlear cells

Tamás, Andrea and Horváth, Gabriella and Reglődi, Dóra and Kiss, Péter and Gasztonyi, Beáta and Tóth, Gábor and Németh, Adrienn and Lujber, László and Pytel, József and Sümegi, Balázs and Rácz, Boglárka (2010) Protective effects of PACAP against oxidative stress in cochlear cells. Journal of Molecular Neuroscience, 42 (3). p. 283. ISSN 0895-8696, ESSN: 1559-1166

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Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide. Numerous studies prove that PACAP has neuroprotective effects in diverse neuronal systems in vitro and in vivo. The involve- ment of PACAP in visual and olfactory sensory processing has also been documented, but little is known about its effects in the auditory system. The presence of PACAP and its receptor, the specific PAC1 receptor, has been shown in the cochlea and in brain structures involved in auditory pathways. The aim of the present study was to investigate whether PACAP is protective in cochlear oxidative stress-induced cell death,which isknown toplaya roleinseveral ototoxicinsults. Chicken cochlear cells were exposed to 1 mM H2O2, which resulted in a marked reduction of cell viability and a parallel increase of apoptotic and necrotic cells assessed by MTT test and flow cytometry. Co-incubation with 100 nM PACAP increased cell viability and reduced the percentage of apoptotic cells. Furthermore, oxidative stress increased the activation of caspase-3, while simultaneous PACAP treatment reduced it. In summary, our present results demonstrate that PACAP effectively protects cochlear cells against oxidative stress-induced apoptotic cell death.

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