REAL

Separation and detection of apricot leaf triterpenes by high-performance thin-layer chromatography combined with direct bioautography and mass spectrometry

Móricz, Ágnes and Ott, Péter (2022) Separation and detection of apricot leaf triterpenes by high-performance thin-layer chromatography combined with direct bioautography and mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1675. No. 463167. ISSN 0021-9673

[img]
Preview
Text
2022_JCA_apricot.pdf
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Prunus armeniaca leaf extract was screened for antibacterial compounds by high-performance thin-layer chromatography (HPTLC)-direct bioautography using a Gram-positive Bacillus subtilis bacterium. Six chromatographic zones exhibited characteristic bioactivity. Five of them also appeared after derivatization with vanillin-sulfuric acid reagent and could be characterized with HPTLC-electrospray ionization (ESI)-mass spectrometry (MS), suggesting the presence of triterpenoids and the fatty acids linolenic and palmitic acid. To confirm the identification of triterpenoids an HPTLC method using in situ pre-chromatographic derivatization with iodine was developed to separate the closely related triterpenoids. After development, the iodine could be eliminated from the chromatogram (verified by HPTLC-MS), making it suitable for the B. subtilis assay. Ursolic acid, oleanolic acid, betulinic acid, corosolic acid, and maslinic acid were discovered for the first time as antibacterial components of P. armeniaca leaves. Their presence was proved also by 2D-HPTLC combined with intermediate in situ derivatization by iodine.

Item Type: Article
Uncontrolled Keywords: Pre-chromatographic derivatization, Two-dimensional HPTLC, HPTLC-Bioassay, 2D-HPTLC-MS, Triterpenoids
Subjects: Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 27 May 2022 08:24
Last Modified: 27 May 2022 08:24
URI: http://real.mtak.hu/id/eprint/143098

Actions (login required)

Edit Item Edit Item