REAL

Interpretation of bafilomycin, pH neutralizing or protease inhibitor treatments in autophagic flux experiments: novel considerations.

Juhász, Gábor (2012) Interpretation of bafilomycin, pH neutralizing or protease inhibitor treatments in autophagic flux experiments: novel considerations. AUTOPHAGY, 8 (12). pp. 1875-6. ISSN 1554-8627

[img]
Preview
Text
2012AUTO0271R.pdf
Available under License Creative Commons Attribution Non-commercial.

Download (666kB) | Preview

Abstract

Recent publications showed that the kinase MTOR localizes to lysosomes and its activation depends on amino acids inside the lysosomal lumen, implying that autophagic protein degradation is a positive regulator of MTOR in this setting. Since decreased MTOR activity results in autophagy induction, drug treatments that block autolysosomal degradation (a commonly used technique to estimate autophagic flux) may actually interfere not only with lysosomal breakdown, but also increase autophagosome generation through impaired MTOR signaling.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: Dr. Gabor Juhasz
Date Deposited: 18 Sep 2014 09:25
Last Modified: 18 Sep 2014 09:25
URI: http://real.mtak.hu/id/eprint/15365

Actions (login required)

Edit Item Edit Item